DNA Topoisomerase Protocols Volume 1: DNA Topology and...

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DNA Topoisomerase Protocols Volume 1: DNA Topology and Enzymes (Methods in Molecular Biology Vol 94)

Name: DNA Topoisomerase Protocols Volume 1: DNA Topology and Enzymes (Methods in Molecular Biology Vol 94)
ISBN: 9780896034440

Bjornsti, Mary-Ann, Osheroff, Neil

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DNA Topoisomerase Protocols is an unprecedented collection of cutting-edge experimental protocols for investigating DNA structure, topology, and DNA topoisomerase function. Described by expert experimentalists who have perfected the techniques, these time-proven methods utilize new approaches to study changes in DNA topology (linking number, knotting, catenation, and relaxation) and DNA structure (bending), as well as to assess chromosome structure through FLP-mediated recombination and to analyze bacterial nucleoid structure. State-of-the-art protocols also detail the expression and purification of bacterial, viral, and eukaryotic DNA topoisomerases from a variety of sources, including baculovirus, bacterial, yeast, and mammalian expression systems.

カテゴリー:

Biology and other natural sciences

年:

2014

出版社:

Springer

言語:

english

ISBN 10:

0896034445

ISBN 13:

9780896034440